Table 1.

Structural parameters from SAXS and MALS data

SAXS sample	Dmax (Å)	Rg (Å) Guinier plot	Rg (Å) from P(r)	MW Seq. Calculated (kDa)	MW SAXS (kDa)	MW MALS (kDa)	Model fit x2
TDP1149–698	∼70	23.1 ± 1.8	23.7	51	40	60	2.9
TDP1	∼110	31.6 ± 0.3	32.3	68	73	70	1.2
TDP1a				68		72
TDP1 + λ phosphatase	∼140	32.0 ± 0.7	33.32	68	64	70	1.1
LigIII170–755-TDP1	∼160	45.0 ± 1.3	48.2	135	140	140	1.9
LigIII170–755-TDP1a				135		124
LigIIIα-TDP1	∼250	65.2 ± 3.0	68.0	172	340	340	1.8
LigIIIα-TDP1a				172		261

^aMolecular mass estimates from SEC and MALS using Superdex 200 column (see Experimental procedures).