Fig. 2.

Loss of JUNB promotes distant metastasis. a Schematic representation of the spontaneous metastasis assay using EO771.LMB tumor cells. b Primary tumor growth of EO771.LMB in CTR and KO mice. Data points represent the mean, error bars SD of all mice of the respective genotype, n = 15 (CTR) and n = 16 (KO). c Macroscopic images of lung metastasis. d Quantification of lung metastatic burden by qPCR of the mCherry reporter compared to vimentin on DNA level. Mann Whitney test p < 0.0001. n = 16 per group. e Representative images of the immunohistochemistry (IHC) for mCherry, scale bar 2 mm, and the quantification thereof (f), n = 12 (CTR) and 13 (KO). Mann Whitney analysis revealed a significant increase of lung metastasis upon deletion of stromal Junb, p = 0.0257. Data in (d) and (f) are displayed as geometric mean with geometric SD, one data point represents one mouse, p. *p < 0.05, ****p < 0.0001