Fig. 5.

Gene expression profiling of FACSorted myeloid cells from tumor-bearing JUNB KO and CTR mice. a Gene expression analysis of mCherry- CD45 + CD3− B220− CD11b + Ly6G^hi neutrophils isolated from early metastatic lungs (upper panel) and tumors (lower panel), n = 4 (lung CTR), n = 5 (lung KO), n = 4 (tumor CTR and KO). b Expression of Mmp9 in alveolar macrophages in the lung (defined as CD45 + CD3− B220− CD11b^low SiglecF^hi CD11c^hi) and macrophages in the primary tumor (identified as mCherry− CD45 + CD3− B220− CD11b + F4/80 + Ly6C−), n = 5 (lung CTR and KO), n = 5 (tumor CTR), n = 4 (tumor KO). c Gene expression analysis of whole lungs from tumor-bearing (EO771.LMB) and non-tumor bearing unchallenged mice, n = 6 (unchallenged CTR + KO), n = 14 (CTR EO771.LMB), n = 18 (KO EO771.LMB). Each data point represents one individual mouse. Data are presented as geometric mean ± geometric SD (a + b) or geometric mean only (c). Statistical analysis (a–c) by Mann Whitney, *p < 0.05, ***p < 0.001, ****p < 0.0001