Fig. 4.

CT-26 cell adhesion and integrin expression. A CT-26 cells were stimulated with CXCL2 and allowed to adhere to wells coated with different ECM proteins as described in Materials and Methods. CT-26 cells were preincubated with vehicle or CXCL2 antagonist (1 μM). B Expression of αV, β1 and β3 integrin subunits on colon cancer cells. Single-cell suspensions were prepared from confluent CT-26 cells and stained as outlined in Materials and Methods. Unstained cells were used as negative control and single tube staining used for each receptor. C CT-26 cells were stimulated with CXCL2 and allowed to adhere to wells coated with different ECM proteins as described in Materials and Methods. CT-26 cells were preincubated with anti-CD51 (αV integrin) antibody 10 μg/ml. Data represents mean ± SEM and n = 4. A ^#P < 0.05 vs untreated and *P < 0.05 vs CXCL2 + Vehicle. B ^#P < 0.05 vs untreated and *P < 0.05 vs CXCL8 + Vehicle