Table 3.

Results of experimental animals inoculated intraduodenally with Clostridium perfringens type D inoculums containing whole culture, culture supernatant, washed cells and culture media.

Treatment group	No. of animals necropsied	Duration of set up of diarrhea	External appearance	Intestine gross lesions	Lung Gross lesions	Isolation of C. perfringens and TmPCR
Group-I (CS)	05	20–24 h	One died after 20 hpi and 02 showed soiled perineal region with diarrhoeic faecal material	Distal portion of ileum mild congested	Emphysema and edema	Isolation- No TmPCR- Yes
Group-II (WC)	05	20–24 h	02 showed soiled perineal region with diarrhoeic faecal material	Distal portion of ileum mild congested	Emphysema and edema	Yes
Group-III (WS)	05	30–36 h	02 showed depression and anorexia	Distal portion of ileum mild congested	Mild congestion	Yes
Group-IV (Control)	03	Apparently normal		Apparently normal		No