Figure 2.

hzVSF does not inhibit viral transcription from cccDNA. a, Time course of the experiment. HepG2 cells were transfected with mcHBV-Gluc (genotype C). After 2 days, baseline glucosidase activities were measured. The indicated compounds were added at 2 and 4 days after transfection. At 6 days after transfection, culture medium was collected for the analysis of Gluc activity. b, Relative glucosidase activities were calculated by treating the values of the negative control samples as 1. Data are presented as the means ± SDs of triplicate experiments. NS, not significant. c, Time course of the experiment. HepG2 cells were transfected with minicircle HBV (genotype D). At 2 and 4 days after transfection, the indicated compounds were added. At 6 days after transfection, cells were harvested, and the 3.5-kb HBV-RNA levels were measured. HBV-RNA levels were calculated by treating the values of the negative control samples as 1. Data are presented as the means ± SDs of triplicate experiments. NS, not significant. ∗p < 0.05.