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hzVSF does not affect viral replication at steps subsequent to cccDNA. a, Time course of the experiment. HepAD38 cells were cultured for 2 days after adding doxycycline (DOX) to stop HBV transcription from the genome. At 3 days after the compounds were added, HBsAg and HBV DNA levels in the culture medium were measured. b, c, HBsAg and HBV DNA levels in the culture medium. Data are presented as the means ± SDs of triplicate experiments. NS, not significant. ∗p < 0.05.
