Fig. 2. VAX1 is required for Kiss1 and Tac2 expression in male ARC.

RT-qPCR profiles of (A,B) Kiss1, (C,D) Tac2, and (E,F) Pdyn in the ARC of intact males and intact diestrus females. Data were analyzed by the 2^-delta delta Ct method and represented as fold change compared to Vax1^WT ± SEM. (G) RT-qPCR of Vax1 in ARC of intact Vax1^WT males, diestrus-staged Vax1^WT females, and OVX+E2 Vax1^WT females. Data were analyzed by the 2^-delta delta Ct method and represented as fold change compared to Male Intact ± SEM. All samples were collected between ZT 4-7. All data were analyzed using (A-F) Student’s t-test or (G) One-way ANOVA. * or different letters indicate significance of p<0.05. N=5-7.