Table 1.
Summary of the 15 differentiation conditions.
| Condition | hPSC line | Seeding density (cells/well) | CHIR99021 concentration | Differentiation efficiency |
|---|---|---|---|---|
| 1.5 m–12 μM | H9 ESC | 1.5 × 106 | 12 μM | 0.3 ± 0.1% |
| 1.5 m–9 μM | H9 ESC | 1.5 × 106 | 9 μM | 0.5 ± 0.2% |
| 100 k–12 μM | H9 ESC | 1.0 × 105 | 12 μM | 0.6 ± 0.3% |
| 19-9-11-600 k–8 μM | 19-9-11 iPSC | 6.0 × 105 | 8 μM | 5.9 ± 0.7% |
| H13-600 k–10 μM | H13 ESC | 6.0 × 105 | 8 μM | 10.8 ± 1.3% |
| 2 m–12 μM | H9 ESC | 2.0 × 106 | 12 μM | 15.1 ± 1.3% |
| 500 k–12 μM | H9 ESC | 5.0 × 105 | 12 μM | 19.6 ± 1.5% |
| 1 m–12 μM | H9 ESC | 1.0 × 106 | 12 μM | 21.7 ± 2.3% |
| IMR90-1 m–12 μM | IMR90-4 iPSC | 1.0 × 106 | 12 μM | 26.4 ± 1.2% |
| IMR90-1 m–9 μM | IMR90-4 iPSC | 1.0 × 106 | 9 μM | 38.5 ± 1.9% |
| 500 K–10 μM | H9 ESC | 5.0 × 105 | 10 μM | 51.8 ± 3.2% |
| IMR90-1 m–10 μM | IMR90-4 iPSC | 1.0 × 106 | 10 μM | 53.2 ± 0.8% |
| 19-9-11-600 k–6 μM | 19-9-11 iPSC | 6.0 × 105 | 6 μM | 61.0 ± 3.2% |
| H13-800 k–10 μM | H13 ESC | 8.0 × 105 | 10 μM | 63.1 ± 2.4% |
| 500 K–9 μM | H9 ESC | 5.0 × 105 | 9 μM | 65.5 ± 2.1% |
hPSCs, including H9 and H13 embryonic stem cells (ESC) or IMR90-4 and 19-9-11 induced pluripotent stem cells (iPSC) were differentiated into CMs following an established method11. H13 ESC, IMR904- iPSC, and 19-9-11 iPSC are specified with H13, IMR90, and 19-9-11. On differentiation day 12, cells were verified by flow cytometry with cTnT labeling from three independent replicates to define differentiation efficiency. Data were collected from three biological replicates. Conditions are presented with condition name (seeding density, CHIR99021 concentration, IMR90 status), hPSC line, seeding density, CHIR99021 (Wnt activator) concentration, and differentiation efficiency (mean ± SEM). Low differentiation efficiencies (< 50% cTnT+ on day 12) are in italic and high differentiation efficiencies (≥ 50% cTnT+ on day 12) are in bold.