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. 2021 Jul 28;12(8):747. doi: 10.1038/s41419-021-04024-9

Fig. 2. Effects of exosomal miR-1260b on migration and invasion of NSCLC.

Fig. 2

A–C A549 cells were transfected with 50 nM control miRNA (NC) or miR-1260b mimic for 48 h following infection of lentiviral shcontrol or shHIPK2. A HIPK2 expression was confirmed by western blotting. B, C Transwell assays were performed to detect changes in migration and invasion abilities. The number of migratory or invading cells was counted for each image field. D, E PC-9 cells were treated with 50 μg of exosomes derived from A549 and their stable cell lines (miR-1260b-O/E or anti-miR-1260b-O/E). HIPK2 expression was confirmed by western blotting, and the ability of migration and invasion was determined using Transwell assays. Data are reported as the mean ± standard deviation of three independent experiments with five fields counted per experiment. F IVIS luciferase in vivo images of lung metastasis. Lung metastasis models by using A549 cells were established as described in “Materials and methods.” Luciferase activities were determined by bioluminescent imaging (BLI) at 2 weeks after injection of the indicated cells. *P < 0.05, **P < 0.005, ***P < 0.0005 compared with the control group.