Table 1.

Speck assay 81,000 high-throughput screening metrics.

Metric	Specification (Spec)	Average (across all plates)	SD
Robust Z′ (positive control, MCC950 5 µM)	> 0.6	0.9	0.1
Robust Z′ (positive control, MCC950 50 nM = IC50)	> 0.2	0.6	0.2
SSMD	> 5	24	13.6
% CV (negative control, maximum speck number)	< 10	5	2.3

Assay plates were subjected to quality control analysis, evaluating the performance of each plate using positive (MCC950 50 nM and 5 µM) and negative (vehicle, DMSO) controls. The quality assay control acceptance criteria selected were robust Z′ > 0.6 (MCC950 5 µM; maximum speck number reduction), robust Z′ > 0.2 (MCC950 50 nM = IC₅₀). SSMD > 5, % CV < 10% (negative control, maximum speck number). Screening metric averages and standard deviations across plates are shown.