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. 2021 Jul 28;11:15313. doi: 10.1038/s41598-021-94500-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of AFL treatment regimens. (a) In the first regimen, mice subjected to the OIR protocol (75% oxygen concentration from P6 to P11) were treated with AFL (25 mg/kg body weight) on P13. Thereafter, on P14, RNA from the retinas of these animals (P14 OIR + AFL) and of non-injected OIR controls (P14 OIR) and normoxic controls (P14 Normoxia; not shown) was collected. (b) In a second treatment regimen, mice subjected to the OIR protocol were treated with AFL on P13 and P15 and RNA from their retinas was isolated on P17 (P17 OIR + AFL). Non-injected age-matched OIR littermates (P17 OIR) and normoxic age-matched animals (P17 Normoxia; not shown) were used as controls. (c) Finally, mice subjected to the OIR protocol received either 2 or 3 doses of AFL (P19 OIR + AFL (× 2) and P19 OIR + AFL (× 3), respectively) prior to RNA collection on P19. Retinas of non-treated age-matched littermates (P19 OIR) and of normoxic age-matched animals (P19 Normoxia) were used as controls.