Fig. 2.

Effect of circ_0014130 silencing in DTX-resistant NSCLC cells in vitro. a–g NCI-H1299/DTX and A549/DTX cells were exogenously administrated with siRNA against circ_0014130 (si-circ_0014130) or the negative control si-NC. a Real-time PCR measured circ_0014130 level after transfection. b, c MTT assay evaluated cell viability of transfected cells after DTX (0–100 μg/L) treatment for 48 h, and the half inhibitory concentration (IC50) of DTX was presented. d Colony formation assay examined relative colony formation (%) after transfection. e Flow cytometry measured the percentage of apoptotic cells after transfection. f, g Transwell assays detected numbers of migratory cells and invasive cells after transfection. Data of each panel were from three independent experiments, and *P < 0.05