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. 2021 Jul 28;4:919. doi: 10.1038/s42003-021-02439-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Overview of MARylated and PARylated protein overlay assays in multiwell plates and detection using MacroGreen fluorescence. The assay consists of (1) the ADP-ribosylation reaction; (2) binding of ADP-ribosylated protein to multiwell plates; (3) binding of MacroGreen to ADP-ribosylation sites; and (4) fluorescence readout using a plate reader. Graphics generated using BioRender.