Skip to main content
. 2021 Jul 28;4:919. doi: 10.1038/s42003-021-02439-w

Fig. 4. MacroGreen can detect ADP-ribosylated sites in fixed and permeabilized cultured cells.

Fig. 4

a MacroGreen staining of DNA damage sites in U2OS DNA double-strand break reporter cells, verified by staining for γH2AX. Control experiments contained a PARP1 inhibitor. Scale bars: Main panel, 10 μm; inset, 2.5 μm. b MacroGreen fluorescence detected after staining of HEK293T cells grown in 96-well plates and treated to induce DNA damage as indicated. n = 12; error bars indicate S.D.