Skip to main content
. 2021 Jul 28;12:4583. doi: 10.1038/s41467-021-24869-0

Fig. 8. Partial rescue of HF phenotype upon VDAC2 reintroduction.

Fig. 8

a Western blot image of VDAC2-GFP and lane-loading control VINCULIN and quantification using Image Studio Lite (version 5.2.5) (KO GFP vs. KO VDAC2-GFP p = 0.0568) (n = 3); b qRT-PCR of Vdac2 normalized to Vcl (Vinculin) (KO GFP vs. KO VDAC2-GFP p = 0.0044) (n = 3); c–g Serial echocardiography parameter including ejection fraction (EF) (week 6: WT GFP vs. KO GFP p = 0.0036, week 10: WT GFP vs. KO GFP p = 0.0008, WT VDAC2-GFP vs. KO VDAC2-GFP p = 0.0456, KO GFP vs. KO VDAC2-GFP p = 0.0359), fractional shortening (FS) (week 6: WT GFP vs. KO GFP p = 0.0091; week 10: WT GFP vs. KO GFP p = 0.0043, WT VDAC2-GFP vs. KO VDAC2-GFP p = 0.0652), left ventricular end diastolic diameter (LVEDD) (pre: WT GFP vs. KO GFP p = 0.0169, WT VDAC2-GFP vs. KO VDAC2-GFP p = 0.0407; week 10: WT GFP vs. KO GFP p = 0.0107, KO GFP vs. KO VDAC2-GFP p = 0.0444), end diastolic and end systolic volumes (EDV (pre: WT GFP vs. KO GFP p = 0.0032; week 10: WT GFP vs. KO GFP p = 0.0104, KO GFP vs. KO VDAC2-GFP p = 0.0378) and ESV (week6: WT GFP vs. KO GFP p = 0.03, week 10: WT GFP vs. KO GFP p = 0.0008, KO GFP vs. KO VDAC2-GFP p = 0.0066)) respectively (n = 3). p-value: unpaired t-test performed in all comparisons between KO GFP and KO VDAC2-GFP in panels a and b. Ordinary one-way ANOVA was performed for echocardiographic data and panel b. Data are represented as ±SEM.