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. 2021 Feb 21;15(8):2233–2247. doi: 10.1038/s41396-021-00919-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Thirteen independent cocultures were subjected to laboratory evolution with and without shaking as described before [5]. DNA samples were collected across generations, end-point-dilutions (EPDs), clonal isolates, and single cells to identify genomic alterations. In addition, clonal isolates were paired in varied combinations in order to determine growth rate and yield for cocultures. Number of samples sequenced are indicated at the bottom.