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. 2021 Jun 18;53:101276. doi: 10.1016/j.molmet.2021.101276

Figure 4.

Figure 4

The gWAT of HFD-fed male Hsp60+/−mice is insulin resistant but shows increased basal glucose uptake. A: Western Blot and densitometric analysis of vena cava insulin-stimulated phosphorylation of AKT1S473 in the gWAT of male Ctrl and Hsp60+/− mice after 16 weeks of HFD. B: Western Blot and densitometric analysis of vena cava insulin-stimulated phosphorylation of AKT1S473 in the scWAT of male Ctrl and Hsp60+/− mice after 16 weeks of HFD. C, D: Basal (C) and maximal (D) respiration of gWAT explants of male Ctrl and Hsp60+/− mice after 16 weeks of HFD. E: Relative light unit detection after ATP-dependent Firefly luciferase assay of lysates from the gWAT of male Ctrl and Hsp60+/− mice after 16 weeks of HFD (N = 12). F: Western Blot and densitometric analysis of GPx1 expression and total protein carbonylation in the gWAT of male Ctrl and Hsp60+/− mice after 16 weeks of HFD. ∗P < 0.05, and ∗∗P < 0.01 after two-tailed Student's t-test. All data are presented as mean ± SEM. The gWAT of HFD-fed male Hsp60+/− mice is insulin resistant but shows increased basal glucose uptake. G: Representative Oil-Red-O staining of differentiated stromal vascular cells derived from the gWAT of male Ctrl and Hsp60+/− mice after 16 weeks of HFD (N = 6). H:14C-Deoxy-d-glucose uptake of gWAT explants from male Ctrl and Hsp60+/− mice after 16 weeks of HFD (N = 5–8). I: mRNA expression of different glucose transporters in the gWAT of male Ctrl and Hsp60+/− mice after 16 weeks of HFD (N = 12). J: Western Blot and densitometric analysis of GLUT8 expression in the gWAT of male Ctrl and Hsp60+/− mice after 16 weeks of HFD. ∗P < 0.05, and ∗∗P < 0.01 after two-tailed Student's t-test. All data are presented as mean ± SEM.