Skip to main content
. 2021 Jun 18;53:101276. doi: 10.1016/j.molmet.2021.101276

Figure 6.

Figure 6

The gWAT of HFD-fed male Hsp60+/−mice shows signs of increased autophagy and lysosomal activity. A: Western Blot and densitometric analysis of LC3 I/II in the gWAT of male Ctrl and Hsp60+/− mice after 16 weeks of HFD. B: Western Blot and densitometric analysis of phosphorylated and total mTOR protein levels in the gWAT of male Ctrl and Hsp60+/− mice after 16 weeks of HFD. C: mRNA expression of HSP60 in 3T3-L1 fibroblasts infected with siRNA targeting either non-target (NT) or Hsp60 (Hsp60 KD). D: Neutral Red absorption in NT or Hsp60 KD 3T3-L1 cells incubated with neutral red for 2 h. E: Lysosomal activity measured via fluorogenic substrate turnover in lysates of gWAT from male Ctrl and Hsp60+/− mice after 16 weeks of HFD (N = 12). ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001 after two-tailed Student's t-test. All data are presented as mean ± SEM.