Table 3.
Primer sequences and target genes used for Q-PCRs.
| Gene | Gene full name | Primer sequence (5′-3′) |
|---|---|---|
| Digestive enzymes | ||
| AMY2A | Pancreatic alpha 2A amylase | F-CGGAGTG↓GATGTTAACGACTGG R-ATGTTCGCAGACCCAGTCATTG |
| PNLIP | Pancreatic lipase | F-GCATCTGGGAAG↓GAACTAGGG R-TGAACCACAAGCATAGCCCA |
| CCK | Cholecystokinin | F-AGGTTCCACTGGGAGGTTCT R-CGCCTGCTGTTCTTTAGGAG |
| Tight junction protein | ||
| Occludin | F-ACGGCAAAGCCAACATCTAC R-ATCCGCCACGTTCTTCAC |
|
| JAM-2 | Junctional adhesion molecules | F-AGACAGGAACAGGCAGTGCT R-TCCAATCCCATTTGAGGCTA |
| Claudin-1 | F-AAGGTGTACGACTCGCTGCT R-CAGCAACAAACACACCAACC |
|
| Gut protective genes | ||
| MUC-2 | Mucin | F-ATTGAAGCCAGCAATGGTGT R-TTGTTGGCCTTGTCATCAAA |
| FABP-6 | Fatty acid-binding protein | F-GAGGACGCACCACGACTAAT R-TTTTCCCACCTTCCATTTTG |
| GLP-2 | Glucagon-like peptide | F-CGTGCCACAGCCATTCTTA R-AGCGGCTCTGCAAATGATTA |
| Housekeeping | ||
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | F-GGTGGTGCTAAGCGTGTTA R-CCCTCCACAATGCCAA |
| TBP | TATA-binding protein | F-GTCCACGGTGAATCTTGGTT R-GCGCAGTAGTACGTGGTTCTC |
AMY2A, pancreatic alpha 2A amylase; PNLIP, pancreatic lipase; CCK, cholecystokinin; JAM, junctional adhesion molecules; glyceraldehyde-3-phosphate dehydrogenase; TBP, TATA-binding protein; MUC-2, mucin-2; FABP-6, fatty acid-binding protein.