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. 2021 Jul 15;12:667644. doi: 10.3389/fphar.2021.667644

FIGURE 11.

FIGURE 11

TFA attenuated podocyte pyroptosis and injury in a METTL3-dependent manner by inhibiting the activation of the NLRP3 inflammasome in HG-afflicted MPC-5 cells with knockdown of METTL3. (A) WB analysis of GSDMD-N, NLRP3, caspase-1, nephrin, and ZO-1, in cultured MPC-5 cells transfected with a NC siRNA or a METTL3 KD siRNA for 24 h, following by exposure to HG at 30 mM for 48 h with or without TFA (20 μg/ml) for 24 h; (B) GSDMD-N was quantified by densitometry; (C) NLRP3 was quantified by densitometry; (D) Caspase-1 was quantified by densitometry; (E) Nephrin was quantified by densitometry; (F) ZO-1 was quantified by densitometry; (G) The cell viability in cultured MPC-5 cells transfected with METTL3 OE vector or METTL3 KD siRNA for 24 h exposed to HG at 30 mM for 48 h. Data are expressed as mean ± S.D., (n = 3). *p < 0.05, **p < 0.01. Abbreviations: TFA, total flavones of Abelmoschus manihot; METTL, methyltransferase-like; NLRP3, NOD-like receptor pyrin domain-containing protein 3; HG, high glucose; MPC-5, mouse podocyte cell-5; WB, western blotting; GSDMD-N, N-terminus of GSDMD; Caspases-1, cysteinyl aspartate-specific proteinase-1; ZO-1, Zonula occludens 1; NC, negative control; siRNA, small interfering RNA; KD, knockdown; OE, overexpression.