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. 2021 Jul 15;12:667644. doi: 10.3389/fphar.2021.667644

FIGURE 2.

FIGURE 2

MCC950 ameliorated podocyte pyroptosis and injury induced by HG in a dose-dependent manner in MPC-5 cells. (A) The cell viability in cultured MPC-5 cells exposed to HG at 30 mM for 48 h with or without MCC950 at 0.1, 1, 10, and 100 μM for 24 h; (B) WB analysis of GSDMD-N, IL-1β, IL-18, nephrin, ZO-1, WT1 and podocalyxin in cultured MPC-5 cells exposed to HG at 30 mM for 48 h with or without MCC950 at 0.1, 1, and 10 μM for 24 h; (C) GSDMD-N was quantified by densitometry; (D) IL-1β was quantified by densitometry; (E) IL-18 was quantified by densitometry; (F) Nephrin was quantified by densitometry; (G) ZO-1 was quantified by densitometry; (H) WT1 was quantified by densitometry; (I) Podocalyxin was quantified by densitometry. Data are expressed as mean ± S.D., (n = 3). *p < 0.05, **p < 0.01 vs. the HG group; & p < 0.05 vs. the TFA (10 μg/ml) group; ## p < 0.01 vs. the TFA (20 μg/ml) group. Abbreviations: HG, high glucose; MPC-5, mouse podocyte cell-5; WB, western blotting; GSDMD-N, N-terminus of GSDMD; IL-1β, interleukin-1β; IL-18, interleukin-18; ZO-1, Zonula occludens 1; WT1, Wilms tumor type 1.