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. 2021 Jul 15;12:667644. doi: 10.3389/fphar.2021.667644

FIGURE 3.

FIGURE 3

TFA and MCC950 attenuated HG-induced inflammasome activation in MPC-5 cells. (A) WB analysis of NEK7, NLRP3, ASC, and caspase-1, in cultured MPC-5 cells exposed to HG at 30 mM for 48 h with or without TFA (20 μg/ml) and MCC950 (10 μM) treatment for 24 h; (B) NEK7 was quantified by densitometry; (C) NLRP3 was quantified by densitometry; (D) ASC was quantified by densitometry; (E) Caspase-1 was quantified by densitometry. Data are expressed as mean ± S.D., (n = 3). *p < 0.05, **p < 0.01. Abbreviations: TFA, total flavones of Abelmoschus manihot; HG, high glucose; MPC-5, mouse podocyte cell-5; WB, western blotting; NEK7, NIMA-related kinase 7; NLRP3, NOD-like receptor pyrin domain-containing protein 3; ASC, a C-terminal caspase recruitment domain; Caspases-1, cysteinyl aspartate-specific proteinase-1.