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. 2021 Jul 15;15:715369. doi: 10.3389/fncir.2021.715369

Figure 4.

Figure 4

Cholinergic and non-cholinergic SOC cells were labeled by retrograde transport from the SOC. (A–C) Examples of ChAT+ tracer-labeled cells in the VNTB ipsilateral to the tracer deposit. Paired photographs show tracer-labeled cells (magenta or green, left panel) and ChAT immunostain (cyan, right panel), demonstrating that the tracer-labeled cells could be ChAT-immunopositive. Examples included cells labeled with green beads (GB) or red beads (RB). (D,E) Examples of ChAT+ tracer-labeled cells in the contralateral lateral superior olivary nucleus [LSO, panel (D) or contralateral VNTB (panel E)]. (F) Example of a GB-labeled ChAT-negative neuron in the LSO ipsilateral to a tracer deposit. LSO, lateral superior olivary nucleus; VNTB, ventral nucleus of the trapezoid body. Panels (A,E, and F) are from deposit G18-2012 GB (medial SOC deposit), panel (B) is from deposit G18-2011 RB (medial SOC deposit), and panels (C) and (D) are from G18-3033 (lateral SOC deposits). Scale bar = 20 μm.