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. 2021 Jul 15;15:715369. doi: 10.3389/fncir.2021.715369

Figure 7.

Figure 7

Cholinergic and non-cholinergic cells in the PMT send branching axonal projections to innervate the SOC bilaterally. (A–C) Each row of photographs shows a single field of view. The first two columns show the tracer label (RB in magenta; GB in green), with the first column showing the tracer injected into the ipsilateral SOC and the second column showing the tracer injected into the contralateral SOC (relative to the labeled cells). Magenta/green arrows identify cells that contain both retrograde tracers. Column 3 shows the ChAT staining, with arrows pointing to the same cells as in the first two columns. Column 4 shows the images merged, highlighting the triple-labeled cells. Examples are from the PPT (A,B) and LDT (C). (D) A single cell in the LDT that contains both retrograde tracers, indicating a bilateral projection to the SOC, but is ChAT-negative (column 3), indicating it is unlikely to be cholinergic. All panels from case G18-2012. Scale bar = 20 μm.