Table 7.

Effects of AT-xynA on relative mRNA expression of ileal tight junction genes in broilers.¹

Items	CON	XY	Value of p
day 7
ZO-1	1.00 ± 0.18	0.80 ± 0.01	0.584
Occludin	1.00 ± 0.17	0.74 ± 0.06	0.458
day 14
ZO-1	1.00 ± 0.13	1.41 ± 0.14	0.169
Occludin	1.00 ± 0.14	1.40 ± 0.11	0.162
day 21
ZO-1	1.00 ± 0.14	1.02 ± 0.12	0.881
Occludin	1.00 ± 0.12	1.00 ± 0.09	0.937
day 42
ZO-1	1.00 ± 0.09	1.11 ± 0.05	0.323
Occludin	1.00 ± 0.06	1.21 ± 0.14	0.438

¹CON is AT-xynA un-supplemented group; XY is 4,000 U/kg AT-xynA supplemented group.

Values are expressed as means ± SEM, n = 6. The values were calculated relative to the expression of β-actin with formula 2^−ΔΔCt. ZO-1, zonula occludens-1.