Figure 1.

DSV induces dose and time-dependent activation of Notch1 signaling markers, SOCS3, and pro-IL-1β expression (A) RAW cells (8x10⁵) were infected with DSV at MOI 5, 20 or 50 for 7 hours. Cells were lysed and protein lysate was prepared. Fifty μg of protein lysate was separated on SDS-PAGE and analyzed for Notch1, NICD, SOCS3, and IL-1β by Western blotting. Actin was used as a loading control. (B–E) Quantification of Western blots. Blots were quantified with ImageJ by analyzing the ratio of protein of interest/Actin. Data represents Mean ± SEM from at least three independent experiments. Values were normalized to control. (F) Cells were infected with DSV (MOI 20) for 0.5, 2, 4, and 7 hours. Fifty μg of protein lysate was separated on SDS-PAGE and analyzed for Notch1, NICD, SOCS3, and IL-1β by Western blotting. Actin was used as a loading control. (G–J) Quantification of Western blots. Blots were quantified with ImageJ by analyzing the ratio of protein of interest/Actin. Values were normalized to control. Data represents Mean ± SEM from at least three independent experiments. One-way ANOVA was used to determine the statistical significance. Values were compared to control, with a post-hoc Dunnett’s test. *p < 0.05, **p < 0.01, ***p < 0.001.