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. 2021 Jul 15;11:695299. doi: 10.3389/fcimb.2021.695299

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Copyright © 2021 Singh, Coffman, Carroll-Portillo, Varga and Lin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DSV differentially induces gene expression of Notch ligands and receptors RAW cells were treated with DSV (MOI 20) for 7hrs and harvested, RNA was isolated and cDNA synthesized. QPCR was carried out to determine the gene expression of (A) DLL1, (B) DLL3, (C) DLL4, (D) Jag1, (E) Jag2, (F) Notch1, (G), Notch2, (H) Notch3, and (I) Notch4 using Taqman probes for respective genes. Relative fold change expression in DSV treated cells compared to untreated control cells was calculated using 2^-ddct method using 18s gene expression as the housekeeping control. A two-tailed t-test was used to determine the statistical difference between control and DSV-treated cells. *p < 0.05, ***p < 0.001.