Figure 3.
DSV-induced Notch signaling, SOCS3, and pro-IL-1β expression is inhibited by gamma secretase inhibitor DAPT (A) RAW cells (8x105) were treated with various concentrations of DAPT (10, 25, and 50 µm in DMSO) overnight. The following day, cells were infected with DSV (MOI 20) for 7 hours. Cells were lysed and protein lysate was prepared. Fifty μg of protein lysate was separated on SDS-PAGE and analyzed for Notch1, NICD, SOCS3, IL-1β, and p21 by Western blotting. Actin was used as a loading control. (B–F) Quantification of Western blots. Blots were quantified with ImageJ by analyzing the ratio of protein of interest/Actin. Data represents Mean ± SEM from at least three independent experiments. One-way ANOVA was used to determine the statistical significance. Values were compared to control, with a post-hoc Dunnett’s test. **p < 0.01 and ***p < 0.001.