Figure 4.

DSV-induced Notch signaling, SOCS3, and IL-1β expression is inhibited by Notch1siRNA (A) RAW cells were transfected with either scrambled (Scr) or siRNA against Notch1 (siNotch1) by electroporation. After incubation for 24 hours, cells were removed and re-plated at a density of 8x10⁵ and further incubated for 24 hours. Following day (total of 48 hrs transfection), cells were infected with DSV (MOI 20) for 7 hours. Cells were lysed and protein lysate was prepared. Fifty μg of protein lysate was separated on SDS-PAGE and analyzed for Notch1, NICD, SOCS3, IL-1β, and p21 by Western blotting. Actin was used as a loading control. (B–F) Quantification of Western blots. Blots were quantified with ImageJ by analyzing the ratio of protein of interest/Actin. Data represents Mean ± SEM from at least three independent experiments. One-way ANOVA was used to determine the statistical significance. Values were compared to Scr+ DSV, with a post-hoc Dunnett’s test. *p < 0.05, **p < 0.01.