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. 2021 Jul 15;11:695299. doi: 10.3389/fcimb.2021.695299

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Copyright © 2021 Singh, Coffman, Carroll-Portillo, Varga and Lin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DSV induces Notch activation in epithelial cells (A) HCT116 cells (8x10⁵) were infected with DSV at MOI 20 for 7 hours. Cells were lysed and protein lysate was prepared. Fifty μg of protein lysate was separated on SDS-PAGE and analyzed for NICD and Actin by Western blotting. (B) Quantification of Western blots. Blots were quantified with ImageJ by analyzing the ratio of NICD/Actin. Data represents Mean ± SEM from at least three independent experiments. Values were normalized to control. **p < 0.01.