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. 2021 Jul 15;11:695299. doi: 10.3389/fcimb.2021.695299

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Copyright © 2021 Singh, Coffman, Carroll-Portillo, Varga and Lin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DSV induces NICD activation in the mouse small intestine Mice were orally gavaged with either PBS or DSV (10⁹). One hour later, mice were euthanized and 1/3^rd parts of small intestinal tissue corresponding to Prox (duodenum), Mid (Jejunum), and Distal (ileum) regions were collected in Trizol. Protein samples (10µg) were prepared and analyzed by Western blotting for NICD expression. Actin was used as a loading control. (A) NICD expression in protein samples from 4 control and 5 DSV-treated tissues (B–D) Quantification of Western blots. Blots were quantified with ImageJ by analyzing the ratio of NICD/Actin. Data represents Mean ± SEM. **p < 0.01, Ϯp > 0.05.