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. 2021 Jul 20;2(3):100641. doi: 10.1016/j.xpro.2021.100641

Figure 1.

Figure 1

The sequence of the TLR7 transcript surrounding the location of SNP rs3853839 is displayed

The transcript information was obtained from the Ensembl Website (Yates et al., 2019). The Figure was adapted from a sequence and primer alignment that was done in CLC Main Workbench (Version 7.9.1).

(A) The SNP Type primers (STA primer and LSP) and the Delta Gene primers are aligned and the outer most primer pair is identified for the preamplification step.

(B) The Delta Gene primers are used for gene expression analysis.

(C and D) (C) ASP1 detects the major allele (C) and (D) ASP2 detects the minor allele (G) during the SNP Typing. If there is no splicing site in the sequence (which is the case for the displayed TLR7 sequence) the same SNP Type primers can be employed for SNP typing of mRNA and gDNA. DG = Delta Gene; STA = specific target amplification; LSP = locus-specific primer; ASP = allele-specific primer; FV = forward; RV = reverse, SNP = single-nucleotide polymorphism.