Name | Volume per tube [μL] | Needed per run [μL] | Per aliquot (with overage) [μL] | Overage Amount [μL] | # Of tubes prepared |
---|---|---|---|---|---|
Single Cell Stop Solution | 50 | 1.94 | 2.5 | 0.56 | 18 |
Single Cell Pre-Amp Mix | 265 | 12.0 | 14.0 | 2.0 | 18 |
Single Cell SuperScript RT | 75 | 3.62 | 4.0 | 0.38 | 18 |
Single Cell VILO RT Mix | 150 | 5.84 | 7.0 | 1.16 | 18 |
Single Cell Dnase I | 50 | 1.4 | 2.5 | 1.1 | 18 |
Single Cell Lysis Solution | 500 | 12.75 | 15.0 | 2.25 | 18 |