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. 2021 Jul 15;12:705826. doi: 10.3389/fmicb.2021.705826

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Copyright © 2021 Shao, Gao, Warren and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Photomicrographs of Quadristicha subtropica n. sp. from life (A–E) and after protargol staining (F–K). (A,B) Ventral views of representative individuals to show different body shapes. (C) Ventral view, to denote contractile vacuole (arrow). (D) Resting cyst. (E) Crystals. (F,H) Ventral views to demonstrate the ciliature. Arrowhead marks the buccal cirrus; circle in F and H demonstrates the postoral ventral and the frontoventral cirri, respectively. (I) Ventral view of posterior end, to show transverse cirri (circle). (G,J,K) Dorsal views to demonstrate the dorsal kineties, caudal cirri, and nuclear apparatus. Arrows in G show the gap in dorsal kinety 1. 1–4, dorsal kineties 1–4. Bars: 50 μm (A–C); 30 μm (D,H,J); 20 μm (E); 70 μm (F).