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. 2021 Jul 12;6(29):18745–18755. doi: 10.1021/acsomega.1c01795

Figure 4.

Figure 4

SSD alone increased expressions of several apoptosis- and autophagy-related proteins. SGC-7901 (A) and SGC-7901/DDP cells (E) were treated with SSD at the indicated concentrations for 48 h, and the expression levels of proteins were detected by Western blot. Lanes of Western blot were measured by the densitometer. Level of cleaved caspase 3 was normalized to the level of pro-caspase 3 in SGC-7901 (B) and SGC-7901/DDP (F), respectively. Expression of p-IκB α was normalized to the expression of total IκB α in SGC-7901 (C) and SGC-7901/DDP (G), respectively. Levels of LC3 B, IKK β, and NF-κB p65 were normalized to β-actin levels in SGC-7901 (D) and SGC-7901/DDP (H), respectively. SGC-7901 (I) and SGC-7901/DDP (K) cells were treated with SSD at a concentration of 2.5 and 5 μg/mL, respectively, for the indicated time. Western blot images were quantified by densitometric analysis, and levels of p62 were normalized to β-actin levels in SGC-7901 (J) and SGC-7901/DDP (L), respectively. *p < 0.05, **p < 0.01, compared with control.