Figure 7.

1Aa promotes synapse regeneration in vitro. (A) Immunohistochemistry of untreated control (KA-), control treated with KA (KA+), explant treated with KA+DHF (DHF), explant treated with KA+1Aa (1Aa), and explant treated with KA and 1Aa+DHF (1Aa+DHF). Hair cells were labeled with Myo7A (white), presynaptic ribbon synapse was labeled with CtBP2 (red), and postsynaptic ribbon synapse was stained with PSD95 (green). Images are representative of four independent experiments. Scale bar represents 10 μm. (B) Quantification of number of synapses per inner hair cell (^** represents p ≤ 0.01, n.s., not significant).