Figure 8.

Ris-1Aa promotes synapse regeneration in vitro. (A) Immunohistochemistry of untreated control (KA-), control treated with KA (KA+), explant treated with KA+ 1Aa, explant treated with KA+ Ris, and explant treated with KA and Ris-1Aa. Hair cells were labeled with Myo7A (white), presynaptic ribbon synapse was labeled with CtBP2 (red), and postsynaptic ribbon synapse was stained with PSD95 (green). Images are representative of 10 independent experiments. Scale bar represents 10 μm. (B) Quantification of synapses per inner hair cell for various treatment conditions (^****p ≤ 0.0001, denotes that counted synapses in both KA- and KA+ were statistically significant relative to all other samples; ^**p ≤ 0.01).