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Loss of BMPR1a in myoepithelium resulted in an upregulation of Spp1. (A) qRT-PCR analysis of Fgf2, Hgf, Nrg1, and Spp1 in sorted mammary myoepithelial cells from control and cKO mice at pregnancy day 14.5. n ≥ 4 biological replicates. (B) qRT-PCR analysis of Spp1 in sorted myoepithelial and luminal cells from wild-type mice at virginal stage and pregnancy day 14.5 (P14.5). n ≥ 3 biological replicates. Data were presented as means ± SD. ∗∗p < 0.01, ∗∗∗p < 0.001.
