Fig. 4.

Hhex inhibited RHOA and CDC42 activation. a pcDNA3.1 or pcDNA3.1-HHEX plasmid was transfected into A549 cells and H1299 cells for 24 h. RHOA activation assay kit was used for detecting RHOA-GTP active form. The kit supplies His-RHOA protein as a control. b H1299 cells were transfected with pcDNA3.1 or pcDNA3.1-HHEX plasmid respectively for 24 h. Then, active CDC42-GTP level was tested by CDC42 activation assay kit. c Hhex was knocked down in A549 cells, RHOA-GTP assay was conducted. d Hhex was knocked down in A549 cells, CDC42-GTP assay was performed. Results are from one representative experiment of at least three