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. 2021 Mar 9;144(6):1711–1726. doi: 10.1093/brain/awab086

Figure 4.

Figure 4

Silent cold-sensing neurons express peptidergic nociceptor molecular markers Nav1.8 and CGRPα. (A) Cartoon (left) of breeding strategy used to generate GCaMP3 reporter mice for each subset of interest. Bar plot (right) showing overlap of reporter expression for each marker with functionally defined cold-sensing neurons. TrkB-CreERT2 (Ntrk2): nveh = 14 from two mice (one male and one female), noxa = 112 from three mice (two males and one female). Calb1-Cre (Calb1): nveh = 7 from one mouse (one male), noxa = 15 from two mice (two females). Trpv1-Cre (Trpv1): nveh = 4 from one mouse (one male), noxa = 87 from two mice (one male and one female). Nav1.8-Cre (Scn10a): nveh = 14 from four mice (two males and two females), noxa = 108 from six mice (four males and two females). Tmem45b-Cre (Tmem45b): nveh = 40 from one mouse (one male), noxa = 36 from three mice (two males and one female). CGRPα-CreERT2 (Calca): nveh = 45 from three mice (one male and two females), noxa = 122 from two mice (one male and one female). (B) Example images (left) and histograms (right) showing overlap of Nav1.8-Cre-dependent tdTomato expression with cold-sensing neurons of different sizes in vehicle- and oxaliplatin-treated mice. Same data as in A. (C) Histogram (left) and bar plot (right) showing overlap of Nav1.8-Cre-dependent tdTomato expression with different types of cold-sensing neurons in PNL-operated mice. n = 57 cells from two mice (one male and one female). (D) Histograms (left) and bar plot (right) showing overlap of Nav1.8-Cre-dependent tdTomato expression with basally active and silent cold-sensing neurons in mice treated with P-CTX-2. n = 56 cells from four mice (one male and three females). (E) Example images (left) and histograms (right) showing overlap of CGRPα-CreERT2-dependent tdTomato expression with cold-sensing neurons of different sizes in vehicle- and oxaliplatin-treated mice. Same data as in A. (F) Histogram (left) and bar plot (right) showing overlap of CGRPα-CreERT2-dependent tdTomato expression with different types of cold-sensing neurons in PNL-operated mice. n = 16 cells from one mouse (one male). (G) Histograms (left) and bar plot (right) showing overlap of CGRPα-CreERT2-dependent tdTomato expression with basally active and silent cold-sensing neurons in mice treated with P-CTX-2. n = 56 cells from two mice (one male and one female). Error bars denote 95% confidence intervals. As these data were obtained as part of an exploratory screen, no statistical hypothesis testing was performed.