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. 2021 Jul 28;9(7):e002528. doi: 10.1136/jitc-2021-002528

Figure 2.

Figure 2

MYC impairs T cell infiltration and cytolytic activity in vivo. (A) Tumor volumes of 66cl4-shNC and 66cl4-shMYC subcutaneous tumors in BALB/c mice (27 days; n=4), as measured using calipers. (B) Tumor weight of 66cl4-shNC and 66cl4-shMYC subcutaneous tumors in BALB/c mice at the time of sacrifice. (C) Representative images of immunohistochemical staining for CD3, CD8 and granzyme B (left), quantification of CD3+, CD8+ and granzyme B+ cells (right) in 66cl4-shNC or 66cl4-shMYC subcutaneous tumors. (D) Tumor volume of 4T1-MYC-vec and 4T1-MYC-oe subcutaneous tumors in BALB/c mice (18 days; n=6), as measured using calipers. (E) Tumor weight of 4T1-MYC-vec and 4T1-MYC-oe subcutaneous tumors in BALB/c mice at the time of sacrifice. (F) Representative images of immunohistochemical staining for CD3, CD8 and granzyme B (left), quantification of CD3+, CD8+ and granzyme B+ cells (right) in 4T1-MYC-vec and 4T1-MYC-oe subcutaneous tumors. The data are presented as the mean±SEM (A–F); two-tailed unpaired Student’s t test (A–F). *P<0.05; **P<0.01; ***P<0.001. SEM, standard error of mean.