Figure 1.

Diagram of inhibition assay used for quantification of CTS1. Biotinylated monoclonal antibody 2F11 is incubated with patient serum at different dilutions for one hour. After incubation, mixture is added to an ELISA plate that has been coated with rCTS1 and allowed to incubate. If CTS1 antigen and/or anti-CTS1 antibodies are present in the sample being tested, the antibody will be blocked from being able to bind rCTS1 in the ELISA plate, generating low signal. If CTS1 antigen and anti-CTS1 antibodies are not present, the antibody will bind rCTS1 in the ELISA plate, generating high signal. Schematic was created with BioRender.com.