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. 2021 Mar 16;144(6):1670–1683. doi: 10.1093/brain/awab083

Figure 3.

Figure 3

SHH attenuates the pro-inflammatory phenotype of human memory CD4 T cells. (A) CFSE-labelled human CD4 T cells were cultured with anti-CD3/CD28 in the absence (control) or presence of the indicated doses of recombinant human SHH. Proliferation was determined after 5 days by flow cytometry. Data shown are representative of two independent experiments. (B and C) Human CD4+CD45RO+ memory T cells isolated from the blood of healthy donors were cultured for 5 days under Th1-skewing conditions in the absence (control) or presence of 100 ng/ml recombinant human SHH. GM-CSF, IFN-γ and IL-17 production was assessed in memory CD4 T cells by flow cytometry. (B) Representative dot plots are from n = 11 donors and six independent experiments. (C) Each pair represents one donor stimulated without (control, open circles) or with (filled circles) recombinant human SHH. Wilcoxon test was used for comparisons. *P ≤ 0.05 and **P ≤ 0.01.