Figure 5.

Age-dependent phosphorylated α-syn pathology (Ab51253) in the autonomic ganglia and spinal cord of seeded and age-matched control rats. High magnification micrographs of phosphorylated α-syn pathology in the coeliac ganglia (A) and cervical ganglia (B) at 10 and 20 weeks post injection (p.i.). Scale bar = 20 µm. α-Syn pathology is age-dependent and progressive. Aged rats seeded with hPFF show significantly more pathology than young and age-matched control rats. Young rats seeded with mPFF exhibit a similar level of pathology compared to old rats seeded with hPFF. (C) Co-localization of α-syn pathology and VAChT (Ab235201) staining in the intermediolateral nucleus of the spinal cord. α-Syn pathology is age-dependent and is absent in young rats seeded with hPFFs and age-matched controls, but not in young rats seeded with mPFFs. The VAChT distribution is similar across experimental groups. Scale bars = 50 µm and 500 µm.