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. 2021 Jul 15;12:669787. doi: 10.3389/fimmu.2021.669787

Figure 3.

Figure 3

IL-33 administration alleviates intestinal inflammation. To induce intestinal inflammation, BALB/c mice (n = 7-13 mice per group) were given DSS via the drinking water for 6 days, followed by one day of normal drinking water. (A) On day 0, 2 and 5 control mice as well as DSS mice were i.p. injected either with recombinant mIL-33 or with PBS. (B) Body weight loss and (C) disease activity index were monitored daily. (D) Histopathological scores of PBS and IL-33-treated DSS-mice were determined on day 7 (n = 5 – 13 mice per group). (E) Representative Hematoxylin and Eosin (H&E) stained colon sections at day 7. Scale bars represent 100 µm. (F) Cytokine levels of IL-33, TNF-α, IL-6, IL-5 and IL-13 were measured in the supernatants of colonic explants by Luminex technology. All data are presented as mean ± SEM. Statistical analyses were performed using two-way ANOVA followed by Tukey’s multiple comparison test (B, C), one-way ANOVA followed by Dunn’s multiple comparison test (F), or using Mann-Whitney test (D). *P < 0.05; **P < 0.01; ***P < 0.001.