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. 2021 Jul 15;9:614324. doi: 10.3389/fbioe.2021.614324

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Copyright © 2021 Grzesik and Warth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cryopreserved CD3⁺ T cells purified from human donors (n = 4) were thawed and activated using anti-CD3/anti-CD28-coupled magnetic beads in test media or reference media in the presence of IL-7 and IL-15 (10 ng/ml, CellGenix) in three technical replicates. On day 3 cells were labeled with 7-AAD and cell viability was determined (upper panel). On day 6 cell counts were determined by flow cytometry and expansion was calculated (lower panel). Symbols indicate replicate values of individual donors.