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. 2021 Jul 15;11:656804. doi: 10.3389/fonc.2021.656804

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Copyright © 2021 Haritha, Nawab, Vijayakurup, Anto, Liju, Alex, Amrutha, Aiswarya, Swetha, Vinod, Sundaram, Guijarro, Herlevich, Krishna, Nestory, Bava, Sadasivan, Zajac-Kaye and Anto

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the efficiency of lentiviral transduction and expression status of TS in transduced cells. (A) Efficiency of lentiviral transfection in HEK 293T cells and successive infection in MDA-MB-231 cells, respectively as assessed by GFP expression. (B) Time-dependent analysis of expression status of TS in MDA-MB-231 cells transduced with TS shRNA compared to that of untreated control and cells transduced with control/non-targeting shRNA (Nt-shRNA) using immunoblotting. GAPDH levels are shown as the loading control. (C, D) Comparison of body weight from different treatment groups bearing MDA-MB-231^TS xenografts and MDA-MB-231^TS- xenografts, respectively.