Figure 5.

The role of TS in regulating the synergism of 5-FU and curcumin. [A (i, ii)] Representative images of animals from each experimental group, bearing tumor produced by orthotopic injection of both sets of transduced cells and tumors excised showed a reduction in tumor size upon completion of treatment. (B, C) Graphs showing a comparison of tumor volume of MDA-MB-231^TS and MDA-MB-231^TS- xenografts, respectively upon completion of treatment. Significant reduction in tumor volume is observed in animals bearing MDA-MB-231^TS xenografts, upon treatment with combination while no significant reduction in tumor volume is observed in animals bearing MDA-MB-231^TS- xenografts. Data represent two independent sets of experiments and results are shown as the mean ± S.D. P-values were calculated with one-way ANOVA. ***P-values ≤0.001, **P-values ≤0.01 and *P-values ≤0.05; ns represents non-significance. [D (i, ii)] Immunohistochemical analysis of expression status of TS and p65 sub-unit of NF-κB in different treatment groups of MDA-MB-231^TS and MDA-MB-231^TS- xenografts, respectively.