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SRF binding to smooth muscle MHC and α-actin promoters decreases during the cell proliferation phase and increases during the cell hypertrophy phase. ChIP was carried out with anti-SRF antibodies, and the promoter regions of smooth muscle (A) MHC and (B) α-actin were amplified and quantitated by real-time PCR (n = 3, mean ± SE, *P < 0.05 compared to corresponding sham-operated time points).
